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anti mouse rat rage antibody  (R&D Systems)


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    R&D Systems anti mouse rat rage antibody
    Anti Mouse Rat Rage Antibody, supplied by R&D Systems, used in various techniques. Bioz Stars score: 95/100, based on 96 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/anti+mouse+rat+rage+antibody/Mouse%2FRat+RAGE+Antibody/pm42014719-313-4-7
    Average 95 stars, based on 96 article reviews
    anti mouse rat rage antibody - by Bioz Stars, 2026-09
    95/100 stars

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    Related Articles

    other:

    Article Title: Estrogen replacement therapy in diabetic ovariectomized female rats potentiates postischemic leukocyte adhesion in cerebral venules via a RAGE-related process
    Article Snippet: The blots were hybridized with anti-mouse/rat RAGE antibody (R&D, 1:500) overnight at 4°C and then 60 min with donkey anti-rat secondary infrared fluorescent antibody (800 nm, 1:5,000, Rockland) and scanned at 800 nm.

    Immunostaining:

    Article Title: Cell fate specification during respiratory development requires ARID1A-containing canonical BAF complex activity.
    Article Snippet: .. Primary antibodies used included anti-mouse/rat RAGE antibody (R&D Systems, MAB1179 – 1:100) and anti-guinea pig SFTPC (Seven Hills Bioreagents, G992 - 1:100) along with secondary antibodies diluted to 1:200 used for immunostaining of tissue sections described above. .. For whole-mount EdU staining, the ClickiT EdU Cell Proliferation Kit for Imaging, Alexa Fluor 647 dye (Invitrogen, C10340) was used in conjunction with the protocol for whole-mount immunohistochemistry as previously described45.

    Article Title: Cell fate specification during respiratory development requires ARID1A-containing canonical BAF complex activity
    Article Snippet: .. Primary antibodies used included anti-mouse/rat RAGE antibody (R&D Systems, MAB1179 – 1:100), anti-guinea pig SFTPC (Seven Hills Bioreagents, G992 - 1:100), and anti-guinea pig TTF1/Nkx2.1 (Seven Hills Bioreagents, G237 - 1:100) along with secondary antibodies diluted to 1:200 used for immunostaining of tissue sections described above. .. For whole-mount EdU staining, the Click-iT EdU Cell Proflieration Kit for Imaging, Alexa Fluor 647 dye (Invitrogen, C10340) was used in conjunction with the protocol for whole-mount immunohistochemistry as previously described .

    Blocking Assay:

    Article Title: Estrogen replacement therapy in diabetic ovariectomized female rats potentiates postischemic leukocyte adhesion in cerebral venules via a RAGE-related process
    Article Snippet: .. The blot was blocked with 0.5× Odyssey blocking buffer for 1 h and then hybridized with anti-mouse/rat RAGE antibody (diluted 1 to 3,000 in the blocking buffer; R&D Systems, Minneapolis, MN) at 4°C overnight. .. After being washed several times with PBS plus 0.5% Tween 20 (PBST), the blot was hybridized with an anti-rat IgG antibody conjugated with infrared fluorescent dye (donkey anti-rat IgG IRDye 800 from Rockland Immunochemicals, Gilbertsville, PA, at 1 to 4,000 dilution in the blocking buffer).



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    Immunoproteasome is required for aged AT2 cell regenerative decline and the establishment of IFN γ+ T cells in tertiary lymphoid structures. A) Schematic figure to show the culture of alveolar organoids from young or aged immunoproteasome knockout (IP KO) and control C57BL/6 (WT) mice in feeder-free media at 7 days of growth, including B) organoid forming efficiency (OFE) and (C) diameter. D) RTqPCR of the IFNγ-inducible MHC-I complex component B2m in isolated lung AT2 cells from young or aged immunoproteasome KO and WT mice. E) Representative immunofluorescence staining of Ki-67 to mark proliferating cells with AT2 cell marker SPC and AT1 cell <t>marker</t> <t>Rage/Ager</t> in corresponding feeder-free organoids from aged mice in A-C. F) The ratio of Ki-67+ proliferating cells in aged immunoproteasome knockout (IP KO) versus WT mice. G) Dysregulated pathways in aged immunoproteasome KO epithelium in comparison to WT based on DEG genes (padj < 0.05, LogFC <0), with bar size representing enrichment score. H) Flow cytometry quantifications of CD8+ and CD4+ (I) T cells as % of all CD45+ immune cells in aged immunoproteasome KO and WT control lungs. J) Zoomed-in multiplex fluorescent images of TLS in aged lungs of immunoproteasome KO and WT controls with K) corresponding absolute quantification of CD8+ T cell and IFNγ+ cell (L) densities. *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001.
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    Immunoproteasome is required for aged AT2 cell regenerative decline and the establishment of IFN γ+ T cells in tertiary lymphoid structures. A) Schematic figure to show the culture of alveolar organoids from young or aged immunoproteasome knockout (IP KO) and control C57BL/6 (WT) mice in feeder-free media at 7 days of growth, including B) organoid forming efficiency (OFE) and (C) diameter. D) RTqPCR of the IFNγ-inducible MHC-I complex component B2m in isolated lung AT2 cells from young or aged immunoproteasome KO and WT mice. E) Representative immunofluorescence staining of Ki-67 to mark proliferating cells with AT2 cell marker SPC and AT1 cell <t>marker</t> <t>Rage/Ager</t> in corresponding feeder-free organoids from aged mice in A-C. F) The ratio of Ki-67+ proliferating cells in aged immunoproteasome knockout (IP KO) versus WT mice. G) Dysregulated pathways in aged immunoproteasome KO epithelium in comparison to WT based on DEG genes (padj < 0.05, LogFC <0), with bar size representing enrichment score. H) Flow cytometry quantifications of CD8+ and CD4+ (I) T cells as % of all CD45+ immune cells in aged immunoproteasome KO and WT control lungs. J) Zoomed-in multiplex fluorescent images of TLS in aged lungs of immunoproteasome KO and WT controls with K) corresponding absolute quantification of CD8+ T cell and IFNγ+ cell (L) densities. *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001.
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    R&D Systems rat anti rage
    Immunoproteasome is required for aged AT2 cell regenerative decline and the establishment of IFN γ+ T cells in tertiary lymphoid structures. A) Schematic figure to show the culture of alveolar organoids from young or aged immunoproteasome knockout (IP KO) and control C57BL/6 (WT) mice in feeder-free media at 7 days of growth, including B) organoid forming efficiency (OFE) and (C) diameter. D) RTqPCR of the IFNγ-inducible MHC-I complex component B2m in isolated lung AT2 cells from young or aged immunoproteasome KO and WT mice. E) Representative immunofluorescence staining of Ki-67 to mark proliferating cells with AT2 cell marker SPC and AT1 cell <t>marker</t> <t>Rage/Ager</t> in corresponding feeder-free organoids from aged mice in A-C. F) The ratio of Ki-67+ proliferating cells in aged immunoproteasome knockout (IP KO) versus WT mice. G) Dysregulated pathways in aged immunoproteasome KO epithelium in comparison to WT based on DEG genes (padj < 0.05, LogFC <0), with bar size representing enrichment score. H) Flow cytometry quantifications of CD8+ and CD4+ (I) T cells as % of all CD45+ immune cells in aged immunoproteasome KO and WT control lungs. J) Zoomed-in multiplex fluorescent images of TLS in aged lungs of immunoproteasome KO and WT controls with K) corresponding absolute quantification of CD8+ T cell and IFNγ+ cell (L) densities. *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001.
    Rat Anti Rage, supplied by R&D Systems, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/anti+mouse+rat+rage+antibody/Mouse%2FRat+RAGE+Antibody/pm41707919-72-32-34
    Average 95 stars, based on 1 article reviews
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    Image Search Results


    Immunoproteasome is required for aged AT2 cell regenerative decline and the establishment of IFN γ+ T cells in tertiary lymphoid structures. A) Schematic figure to show the culture of alveolar organoids from young or aged immunoproteasome knockout (IP KO) and control C57BL/6 (WT) mice in feeder-free media at 7 days of growth, including B) organoid forming efficiency (OFE) and (C) diameter. D) RTqPCR of the IFNγ-inducible MHC-I complex component B2m in isolated lung AT2 cells from young or aged immunoproteasome KO and WT mice. E) Representative immunofluorescence staining of Ki-67 to mark proliferating cells with AT2 cell marker SPC and AT1 cell marker Rage/Ager in corresponding feeder-free organoids from aged mice in A-C. F) The ratio of Ki-67+ proliferating cells in aged immunoproteasome knockout (IP KO) versus WT mice. G) Dysregulated pathways in aged immunoproteasome KO epithelium in comparison to WT based on DEG genes (padj < 0.05, LogFC <0), with bar size representing enrichment score. H) Flow cytometry quantifications of CD8+ and CD4+ (I) T cells as % of all CD45+ immune cells in aged immunoproteasome KO and WT control lungs. J) Zoomed-in multiplex fluorescent images of TLS in aged lungs of immunoproteasome KO and WT controls with K) corresponding absolute quantification of CD8+ T cell and IFNγ+ cell (L) densities. *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001.

    Journal: bioRxiv

    Article Title: Local IFNγ signaling contributes to the regenerative decline of aged alveolar progenitor cells

    doi: 10.64898/2026.04.07.716929

    Figure Lengend Snippet: Immunoproteasome is required for aged AT2 cell regenerative decline and the establishment of IFN γ+ T cells in tertiary lymphoid structures. A) Schematic figure to show the culture of alveolar organoids from young or aged immunoproteasome knockout (IP KO) and control C57BL/6 (WT) mice in feeder-free media at 7 days of growth, including B) organoid forming efficiency (OFE) and (C) diameter. D) RTqPCR of the IFNγ-inducible MHC-I complex component B2m in isolated lung AT2 cells from young or aged immunoproteasome KO and WT mice. E) Representative immunofluorescence staining of Ki-67 to mark proliferating cells with AT2 cell marker SPC and AT1 cell marker Rage/Ager in corresponding feeder-free organoids from aged mice in A-C. F) The ratio of Ki-67+ proliferating cells in aged immunoproteasome knockout (IP KO) versus WT mice. G) Dysregulated pathways in aged immunoproteasome KO epithelium in comparison to WT based on DEG genes (padj < 0.05, LogFC <0), with bar size representing enrichment score. H) Flow cytometry quantifications of CD8+ and CD4+ (I) T cells as % of all CD45+ immune cells in aged immunoproteasome KO and WT control lungs. J) Zoomed-in multiplex fluorescent images of TLS in aged lungs of immunoproteasome KO and WT controls with K) corresponding absolute quantification of CD8+ T cell and IFNγ+ cell (L) densities. *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001.

    Article Snippet: We used the following primary antibodies: α-SPC (1:500, AB3786, Millipore), α-RAGE/AGER (1:500, MAB1179-100, R&D System), α-Ki-67 (1:200, 550609, BD Bioscience).

    Techniques: Knock-Out, Control, Isolation, Immunofluorescence, Staining, Marker, Comparison, Flow Cytometry, Multiplex Assay, Quantitative Proteomics